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KMID : 0365819680080010147
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Journal of Pusan Medical College
1968 Volume.8 No. 1 p.147 ~ p.155
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Histochemistry of the Developing Chick Kidney
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Abstract
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and function of the various structures of the developing chick kidney with accordance of RNA, DNA, glycogen and phospholipid. For the purpose to clarify the above mentioned fact the author observed the mentioned enzyme and other substances by histochemically. As natural the mesonephros and metanephros of 4-20 day white leghorn embryos and the one day chicks after hatching were used.
The results summarized as follows;
1. Before definite appearance of the secretory tubules the alkaline phosphatase activity showed strongly in the undifferentiated mesenchymal tissue. As the tubules grew differentiated the alkaline phosphatase activity was found to have disappeared in the mesenchymal tissue surrounding the tubules.
2. Ribonucleic acid and deoxyribonucleic acid were found relatively increased in the undifferentiated mesenchyme surrounding the nephrogenic cord before the tubules were differentiated.
3. The fact that vicissitude of alkaline phosphatase was found to be coincident with that of both nucleic acids suggests that the enzyme may be concerned with histodifferentiation, protein synthesis and multiplication of cells.
4. The fact the strong alkaline phosphatase activity and the occurance of the PAS positive material were observed at the luminal borders of differentiating secretory tubules of mesonephros and metanephros, indicates that alkaline phosphatase may be concerned with reabsorption of carbohydrate at the borders.
5. Strong positive reaction of phospholipid was fonud in the cytoplasm and the cell membrane of the mesonephrie and and metanephric tubules.
6. Coincident simultaneous distribution of the enzyme (alkaline phosphatase) and the lipid (phospholipid) on the mesonephric and metanephric tubules indicates that the former (alkaline phosphatase) may have influence to the metabolism of the latter (phospholipid).
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